The dental industry is fast in inventing and producing a wide range of dental materials and equipment. Before these materials and technologies can be used for safer purposes, it is the responsibility of dentists to use them. This study aimed to examine cell culture and microscopy as research aids in conservative dentistry and endodontics. Compared to other dental professions, conservative dentistry, and endodontics have outpaced advancements in this area due to the rapid growth of several materials and techniques. The field has progressed, yet the extrapolation of outcomes and methodologies for application has been contested. Microbiological aids, instrumental aids, and microscopy all contribute to the diffusion of information, in addition to creative designs and techniques, for the betterment of the profession and professionals. This makes excellent studies accessible to all through publications and leads to changes in notions and attitudes. The basis of the subject is built by study aids, which will influence the future use of any substance, method, or therapy.