This study investigated whether treating human coronal dentin with DMSO influences the adhesion of AH26 sealer. Seventy dentin samples were prepared and split evenly into two groups. All specimens underwent surface conditioning with 2 mL of 2.5 percent NaOCl, 3 mL of 17% EDTA, and a distilled water rinse. One group received an additional final rinse with 50% DMSO before AH26 application. Shear bond strength was then assessed using a universal testing machine at a crosshead speed of 0.5 mm/min. Statistical analysis using a paired samples t-test showed no significant difference between the groups, demonstrating that DMSO treatment did not affect AH26 bonding to dentin.