Compliance with the principles and methods of professional commitment affects the therapist-patient relationship and is of great importance. This study was conducted to investigate professional commitment in dental students and assistants. In this descriptive cross-sectional study, 101 final-year students and 52 specialized assistants participated in the available sample method. The data collection tool was a researcher-made questionnaire taken from the ADEA Tool for Action on Professionalism in Dental Education. After designing the tool, its face and content validity and reliability were evaluated and confirmed. Kruskal-Wallis and Mann-Whitney tests were used for data analysis with P < 0.05. The average score of students' views on professional commitment was 115.61 ± 12.23 (out of a total of 145 scores) and that of assistants was 120.17 ± 7.00 (P = 0.04 and Z = 2.02). The scores under the adequacy and responsibility scales were higher in assistants than in students. The average adequacy score of students was 27.45±2.93, and assistants were 28.69 ± 1.76 (P = 0.006 and Z = 2.7), and the average responsibility score of students was 19.22 ± 2.82, and assistants was 20.42 ± 1.78 (P = 0.008 and Z = 0.51). Female assistants had higher scores than male assistants in the total score and responsibility subscale. According to the obtained results, both groups of general students and specialized dental assistants had a high mean score in the perspective of professional commitment. Because in some areas students have obtained a lower grade, to improve the quality of education and evaluation, professional commitment should be considered in the course plan.
